By Afaf Kamal-Eldin, Jan Pokorny
Lipid oxidation, even though researched because the starting of the twentieth century, nonetheless offers no whole and passable details at the composition of oxidized lipids. One vital issue contributing to those gaps in our wisdom approximately lipid oxidation pertains to the shortages in analytical technique. Analytical tools compatible for oxidized lipids have been usually reviewed within the final decade, yet typically from the element of choice of person oxidized lipid periods, corresponding to peroxides, aldehydes, polar lipids, or polymers. during this publication, they're taken care of from the viewpoint of sorts of analytical equipment used, together with diversified volumetric equipment, UV-visible spectrometric equipment, excessive functionality size-exclusion chromatography, nuclear magnetic resonance spectroscopy, electron spin resonance spectroscopy, and differential scanning calorimetry. research of Lipid Oxidation is key for additional advancements in analytical technique and hyphenated concepts, with which extra knowing of the response kinetics, mechanism, and implications will occur.
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Additional info for Analysis of lipid oxidation
2003) because it is a good solvent for both lipids and DNPH. The DNPH formed by the reaction of 2,4-dinitrophenylhydrazine with lipid oxidation products may be separated by HPLC, and the absorbance of the DNPH peaks can be measured. Both polar and nonpolar reaction products may be separated from one another, and the individual DNPH determined (Seppanen and Csallany 2001). We think this is the best way to utilize the advantages of the method because carbonyl compounds are better identified in the presence of other oxidation products.
HPSEC analysis requires a total run time of only 15 min. Concentrations are calculated from peak areas and gravimetric determination of the polar fraction. The methodology was recently standardized by the IUPAC, with slight modifications (Dobarganes et al. 2000) for the analysis of virgin and refined oils, and for used frying fats. , nonpolar or nonoxidized triacylglycerols (TG), application of HPSEC to the concentrated fraction of polar compounds allows the separation and quantitation of different groups of oxidized and hydrolytic compounds that differ substantially in MW: TG polymers (TGP), TG dimers (TGD), oxidized TG monomers (oxTGM), diacylglycerols (DG), monoacylglycerols (MG), and free fatty acids (FFA), in that order of elution.
And Tzia, C. (2002) A Kinetic Study of Oil Deterioration During Frying and a Comparison with Heating, J. Am. Oil Chem. Soc. 79, 133–137. , and Korenaga, T. (1992) Flow-Injection Analysis for Malondialdehyde in Plasma with the Thiobarbituric Acid Reaction, Clin. Chem. 30, 2061–2062. , and Goto, H. (1969) Estimation of Oil and Fat Peroxide Values, Nippon Daigaku Yakugaku Kenkyu Hokoku 10, 21–24. A. (1993) Evaluation of Oxidized Lipids in Foods, INFORM 4, 811–819. , and Pokorn´y, J. (1959) Colorimetric Determination of Fat Peroxides by Means of Titanium Chloride, Sb.
Analysis of lipid oxidation by Afaf Kamal-Eldin, Jan Pokorny