By S. Suzanne Nielsen
This moment variation laboratory handbook used to be written to accompany nutrition research, Fourth version, ISBN 978-1-4419-1477-4, by way of an identical writer. The 21 laboratory workouts within the guide conceal 20 of the 32 chapters within the textbook. a number of the laboratory routines have a number of sections to hide a number of tools of research for a selected meals component to attribute. lots of the laboratory workouts comprise the subsequent: creation, analyzing task, aim, precept of approach, chemical substances, reagents, precautions and waste disposal, provides, apparatus, approach, facts and calculations, questions, and references. This laboratory guide is perfect for the laboratory part of undergraduate classes in foodstuff research.
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Additional info for Food Analysis Laboratory Manual
H2SO4) Kjeldahl digestion tablets Potassium sulfate (K2SO4) Cupric sulfate Titanium dioxide (TiO2) Tris (hydroxymethyl) aminomethane (THAM) CAS No. Hazards 10043-35-3 76-60-8 64-17-5 7647-01-0 Highly flammable Corrosive 493-52-7 1310-73-2 7664-93-9 77-86-1 Corrosive Corrosive Irritant 7778-80-5 7758-98-7 13463-67-7 Irritant Reagents Notes Both the Kjeldahl and nitrogen combustion methods can be done without automated instrumentation, but are commonly done with automated instruments. The descriptions below are based on the availability of such automated instrumentation.
The Kjeldahl procedure can be basically divided into three parts: (1) digestion, (2) distillation, (3) titration. In the digestion step, organic nitrogen is converted to an ammonium in the presence of a catalyst at approximately 370°C. In the distillation step, the digested sample is made alkaline with NaOH and the nitrogen is distilled off as NH3. This NH3 is “trapped” in a boric acid solution. The amount of ammonia nitrogen in this solution is quantified by titration with a standard HCl solution.
What were the advantages of using the Goldfish extraction method rather than the Soxhlet extraction method? 3. If the fat content measured here differed from that reported on the nutrition label, how might this be explained? 34 Chapter 4 METHOD C: MOJONNIER METHOD Principle Fat is extracted with a mixture of ethyl ether and petroleum ether. The extract containing the fat is dried and expressed as percent fat by weight. The assay uses not only ethyl ether and petroleum ether, but also ammonia and ethanol.
Food Analysis Laboratory Manual by S. Suzanne Nielsen